Beushausen S, Kladakis A, Jaffe H
Laboratory of Neurobiology, National Institutes for Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
DNA Cell Biol. 1993 Dec;12(10):901-9. doi: 10.1089/dna.1993.12.901.
Multiple transcripts coding for kinesin light chain isoforms are present in the tissues of the squid Loligo pealii. Isoform diversity arises through alternative RNA splicing in the amino and carboxyl termini of the putative proteins. Comparison to rat and Drosophila proteins demonstrates a remarkable conservation of structural domains and regulatory motifs. We have identified a PEST domain that may be the site of degradative uncoupling of kinesin functions. Selective transcript distribution occurs in disparate tissues, suggesting an adaptation toward specialized functions. Expression is highest in the nervous system and some evidence for neural-specific transcripts is provided. In neurons, this may relate to the differential targeting of specific membrane-bound organelles such as synaptic vesicles.
编码驱动蛋白轻链亚型的多种转录本存在于枪乌贼Loligo pealii的组织中。亚型多样性是通过假定蛋白质的氨基和羧基末端的可变RNA剪接产生的。与大鼠和果蝇蛋白质的比较表明结构域和调控基序具有显著的保守性。我们鉴定出一个PEST结构域，它可能是驱动蛋白功能降解解偶联的位点。选择性转录本分布在不同组织中，表明其适应了特殊功能。在神经系统中表达最高，并提供了一些神经特异性转录本的证据。在神经元中，这可能与特定膜结合细胞器（如突触小泡）的差异靶向有关。
