Ohhara M, Kurosu Y, Esumi M
Medical Research Institute, Nihon University School of Medicine, Tokyo, Japan.
Biotechniques. 1994 Oct;17(4):726, 728.
We report a simple and rapid method for DNA preparation suitable for PCR by microwave irradiation. When mouse whole blood and hair shafts were directly irradiated and subjected to PCR, a unique gene such as the retinoblastoma susceptibility gene was reproducibly amplified. This method was useful for screening a transgene sequence in transgenic mice.
我们报告了一种通过微波辐射制备适用于聚合酶链反应（PCR）的DNA的简单快速方法。当对小鼠全血和毛发轴进行直接辐射并进行PCR时，诸如视网膜母细胞瘤易感基因等独特基因能够被重复扩增。该方法对于筛选转基因小鼠中的转基因序列很有用。
