Kruk J, Schmid G H, Strzałka K
Department of Plant Physiology and Biochemistry, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Free Radic Res. 1994 Nov-Dec;21(6):409-16. doi: 10.3109/10715769409056593.
Oxidation of biological prenylquinols, like plastoquinol-9 (PQH2-9), ubiquinol-10 (UQH2-10), reduced vitamins K1 (VK1H2) and K2 (VK2H2), alpha-tocopherol quinol (alpha-TQH2) and alpha-tocopherol (alpha-T) was followed by their fluorescence during sonication of egg yolk lecithin/prenylquinol liposomes. The order of magnitude of oxidation of the prenylquinols by free radicals generated during sonication was UQH2-10 > VK2H2 > VK1H2 > alpha-TQH2 > PQH2-9 > alpha-T. It was shown that egg yolk lecithin undergoes degradation even when sonicated briefly under atmosphere of nitrogen and at 0 degree C. A kinetic study of free radical scavenging action of the prenylquinols in solvents of different polarity was performed. The pseudo-first-order rate constants, k, for the reaction of the prenylquinols with 1,1-diphenyl-2-picrylhydrazyl (DPPH) in hexane showed that their scavenging activity changes in the order VK2H2 > VK1H2 > alpha-TQH2 > PQH2-9 > alpha-T > UQH2-10, being the highest in hexane and methanol, whereas in acetone and ethyl acetate the scavenging activity appeared much lower. The reaction rate constants, k, were apparently not dependent on the solvent polarity. The antioxidant activity of the prenylquinols in natural membranes is discussed.
在蛋黄卵磷脂/异戊二烯基醌脂质体超声处理过程中，通过荧光跟踪生物异戊二烯基对苯二酚（如质体醌-9（PQH2-9）、泛醌-10（UQH2-10）、还原型维生素K1（VK1H2）和K2（VK2H2）、α-生育酚对苯二酚（α-TQH2）和α-生育酚（α-T））的氧化。超声处理过程中产生的自由基对异戊二烯基对苯二酚的氧化程度顺序为：UQH2-10 > VK2H2 > VK1H2 > α-TQH2 > PQH2-9 > α-T。结果表明，即使在氮气气氛下于0℃短暂超声处理，蛋黄卵磷脂也会发生降解。对异戊二烯基对苯二酚在不同极性溶剂中的自由基清除作用进行了动力学研究。异戊二烯基对苯二酚与1,1-二苯基-2-苦基肼（DPPH）在己烷中反应的伪一级速率常数k表明，它们的清除活性顺序为VK2H2 > VK1H2 > α-TQH2 > PQH2-9 > α-T > UQH2-10，在己烷和甲醇中最高，而在丙酮和乙酸乙酯中清除活性明显较低。反应速率常数k显然不依赖于溶剂极性。文中讨论了异戊二烯基对苯二酚在天然膜中的抗氧化活性。
