Kruk J, Strzałka K, Leblanc R M
Department of Physiology and Biochemistry of Plants, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
J Photochem Photobiol B. 1993 Jun;19(1):33-8. doi: 10.1016/1011-1344(93)80090-v.
It was found that plastoquinol-9, ubiquinol-10 and alpha-tocopherol quinol show intrinsic fluorescence in organic solvents and in liposomes. Their fluorescence spectra in solution showed the presence of one emission band with maximum intensity in the range 319.0-327.0 nm for plastoquinol and 321.5-326.5 nm for alpha-tocopherol quinol, which is the longest wavelength shifted in polar solvents. The emission band at about 371 nm for ubiquinol was not sensitive to solvent polarity. For all three prenylquinones the fluorescence quantum efficiency changed significantly in solvents of different polarities, being the highest in ethanol and the lowest in hexane in the case of plastoquinol and alpha-tocopherol quinol, whereas ubiquinol fluorescence showed the opposite effect. These spectral parameters were applied to determination of prenylquinol localization in liposome membranes.
研究发现，质体醌 -9、泛醌 -10和α -生育酚醌在有机溶剂和脂质体中呈现内在荧光。它们在溶液中的荧光光谱显示，质体醌有一个发射带，其最大强度在319.0 - 327.0 nm范围内，α -生育酚醌的最大强度在321.5 - 326.5 nm范围内，这是在极性溶剂中波长移动最长的情况。泛醌在约371 nm处的发射带对溶剂极性不敏感。对于所有三种异戊二烯醌，荧光量子效率在不同极性的溶剂中变化显著，就质体醌和α -生育酚醌而言，在乙醇中最高，在己烷中最低，而泛醌荧光则呈现相反的效果。这些光谱参数被用于测定异戊二烯醌在脂质体膜中的定位。
