Boehmelt G, Madruga J, Dörfler P, Briegel K, Schwarz H, Enrietto P J, Zenke M
Institute of Molecular Pathology, Vienna, Austria.
Cell. 1995 Jan 27;80(2):341-52. doi: 10.1016/0092-8674(95)90417-4.
A conditional v-Rel estrogen receptor fusion protein, v-RelER, causes estrogen-dependent but otherwise unaltered v-rel-specific transformation of chicken bone marrow cells. Here, we demonstrate that such v-relER-transformed cells exhibit B lymphoid determinants in line with earlier studies on v-rel-transformed cells. However, following inactivation of v-RelER oncoprotein activity by administration of an estrogen antagonist, cells differentiate into antigen-presenting dendritic cells as judged by several morphological and functional criteria. Additionally, under yet different culture conditions, v-relER cells differentiate into cells resembling polymorphonuclear neutrophils. Our studies therefore suggest that the conditional v-RelER, and probably also the authentic v-Rel, transform a common progenitor for neutrophils and dendritic cells.
一种条件性v-Rel雌激素受体融合蛋白，即v-RelER，可导致鸡骨髓细胞发生雌激素依赖性但其他方面未改变的v-rel特异性转化。在此，我们证明，与早期对v-rel转化细胞的研究一致，此类v-relER转化细胞表现出B淋巴细胞决定簇。然而，在用雌激素拮抗剂使v-RelER癌蛋白活性失活后，根据多种形态学和功能标准判断，细胞分化为抗原呈递树突状细胞。此外，在截然不同的培养条件下，v-relER细胞分化为类似多形核中性粒细胞的细胞。因此，我们的研究表明，条件性v-RelER，可能还有真正的v-Rel，转化了中性粒细胞和树突状细胞的共同祖细胞。
