Walker A K, Enrietto P J
Department of Microbiology, State University of New York at Stony Brook, Stony Brook, NY 11794, USA.
Oncogene. 1996 Jun 20;12(12):2515-25.
The oncogene, v-rel is a member of the rel/NF-kappaB family of transcription factors. It causes a rapidly fatal lymphoma in young chicks and is capable of transforming both fibroblasts and primitive hematopoietic cells in culture. To understand the role of v-rel in transformation we constructed an inducible form of v-rel and used it to identify potential cellular target genes for v-rel regulation. In this paper we show that High Mobility Group Protein 14 (HMG 14) is expressed in a wide variety of v-rel transformed cell types. In addition we show that v-rel participates in the transcriptional regulation of HMG 14 and that extracts from v-relER cells interact with the HMG 14 promoter. These experiments suggest a role for v-rel in the regulation of a unique gene whose protein product may influence gene transcription in a global fashion.
致癌基因v-rel是转录因子rel/NF-κB家族的成员。它会在幼鸡中引发迅速致命的淋巴瘤，并且能够在培养物中转化成纤维细胞和原始造血细胞。为了了解v-rel在转化过程中的作用，我们构建了一种可诱导形式的v-rel，并利用它来鉴定v-rel调控的潜在细胞靶基因。在本文中，我们表明高迁移率族蛋白14（HMG 14）在多种v-rel转化的细胞类型中表达。此外，我们还表明v-rel参与了HMG 14的转录调控，并且来自v-relER细胞的提取物与HMG 14启动子相互作用。这些实验表明v-rel在调控一个独特基因中发挥作用，该基因的蛋白质产物可能以全局方式影响基因转录。
