Jiang M, Liu X
Institute of Acupuncture and Moxibustion, China Academy of T. C. M. Beijing.
Zhen Ci Yan Jiu. 1994;19(1):4-7.
This work was to study the influence of lesion of Sm II on effects of electroacupuncture (EA) upon nucleus raphe magnus (NRM). The experiments were performed on rats. The unit discharges of NRM neurons and their responses to noxious stimulation on tail tip were recorded extracellularly with glass microelectrode. The excitatory neurons of NRM were chosen and divided into two groups, one of them (n = 10) was control before lesion of bilateral Sm II and another (n = 17) after lesion, two neurons of this group were also observed before lesion. It was found that the neurons in the control group before lesion of sm II could be activated by EA of "Zusanli", the increase of spontaneous diacharges in 0-10 minutes and the 20th minute, and the decrease of their nociceptive responses in 0-25 minutes were statistically significant (P < 0.05-0.001). But in the group after lesion the neurons were not obviously activated by EA of "Zusanli". The difference of the spontaneous discharges between the two group was statistically significant in 0-10 minutes (P < 0.05-0.001), the nociceptive responses in 0-10 minutes and at the 20th minute (P < 0.05-0.001). The results show that integrity of Sm II is important to acupuncture analgesia, and further suggest that information from EA of "Zusanli" could arrive at Sm II at least partially, and then activate NRM inducing analgesia.
本研究旨在探讨损毁中缝大核（NRM）对电针（EA）效应的影响。实验在大鼠身上进行。用玻璃微电极细胞外记录NRM神经元的单位放电及其对尾尖有害刺激的反应。选取NRM兴奋性神经元并分为两组，其中一组（n = 10）在双侧中缝大核（Sm II）损毁前作为对照组，另一组（n = 17）在损毁后，该组中有两个神经元在损毁前也进行了观察。结果发现，Sm II损毁前对照组的神经元可被“足三里”电针激活，0 - 10分钟及20分钟时自发放电增加，0 - 25分钟时伤害性反应降低，差异有统计学意义（P < 0.05 - 0.001）。但损毁后组的神经元未被“足三里”电针明显激活。两组在0 - 10分钟时自发放电差异有统计学意义（P < 0.05 - 0.001），在0 - 10分钟及20分钟时伤害性反应差异有统计学意义（P < 0.05 - 0.001）。结果表明，Sm II的完整性对针刺镇痛很重要，并进一步提示“足三里”电针信息至少部分可到达Sm II，进而激活NRM产生镇痛作用。
