Ahmed N, Hale K
Regional Toxicology Laboratory, City Hospital NHS Trust, Birmingham, UK.
Clin Chim Acta. 1994 Oct 31;230(2):201-8. doi: 10.1016/0009-8981(94)90272-0.
An improved capillary gas chromatography method is described for the assay of urinary phenol for the assessment of benzene exposure. Enzymatic hydrolysis with an extract of Helix pomatia is used to liberate phenol from its glucuronide and sulphate conjugates in urine and has the advantage in that complete hydrolysis occurs and clean chromatograms are produced. Furthermore, an internal standard (2,4-xylenol) has been incorporated in the method enabling rapid and single step extraction of phenol. The microassay can be used to measure phenol concentrations as low as 1 mg/l in urine thus making it a suitable method for monitoring benzene exposure.
描述了一种改进的毛细管气相色谱法，用于测定尿酚以评估苯暴露情况。采用来自苹果螺提取物的酶促水解法从尿液中的葡糖醛酸和硫酸酯共轭物中释放出酚，其优点是水解完全且能产生清晰的色谱图。此外，该方法中加入了内标物（2,4-二甲苯酚），可实现酚的快速单步提取。这种微量测定法可用于测量尿液中低至1毫克/升的酚浓度，因此使其成为监测苯暴露的合适方法。
