Lee B L, Ong H Y, Shi C Y, Ong C N
Department of Community Medicine, National University of Singapore, Kent Ridge.
J Chromatogr. 1993 Sep 22;619(2):259-66. doi: 10.1016/0378-4347(93)80115-k.
A method was developed for simultaneous determination of urinary hydroquinone, catechol and phenol using high-performance liquid chromatography (HPLC) with variable-wavelength fluorimetric detection. Urine samples, after acid hydrolysis, were saturated with sodium sulphate and extracted by diethyl ether. The two buffers used for gradient elution were (A) 10 mM sodium acetate containing 0.5% (v/v) acetic acid and (B) the same as buffer A but containing an additional 20% (v/v) acetonitrile. Hydroquinone, catechol and phenol were separated in a C18 column and detected at 2.9, 6.8 and 13.6 min, respectively. The recovery and reproducibility were generally over 90%. Over 300 extracted samples were analysed and no change in column efficiency was noted. Comparisons were also made with HPLC using ultraviolet (UV) detection and with gas chromatography (GC). The proposed method appears to be more sensitive and reliable than other existing methods. This new method was also validated with urine samples collected from cigarette smokers and from refinery workers exposed to low concentrations of benzene.
建立了一种采用高效液相色谱（HPLC）可变波长荧光检测法同时测定尿中对苯二酚、儿茶酚和苯酚的方法。尿样经酸水解后，用硫酸钠饱和，再用乙醚萃取。用于梯度洗脱的两种缓冲液分别为：（A）含0.5%（v/v）乙酸的10 mM乙酸钠溶液；（B）与缓冲液A相同，但额外含有20%（v/v）乙腈的溶液。对苯二酚、儿茶酚和苯酚在C18柱上分离，出峰时间分别为2.9、6.8和13.6分钟。回收率和重现性一般超过90%。分析了300多个萃取样品，未发现柱效有变化。还与采用紫外（UV）检测的HPLC法和气相色谱（GC）法进行了比较。所提出的方法似乎比其他现有方法更灵敏、更可靠。该新方法还通过收集吸烟人员和接触低浓度苯的炼油厂工人的尿样进行了验证。
