Crenesse D, Fossat B, Craffa F, Chaland P, Porthe-Nibelle J, Poiree J C, Gugenheim J
Laboratoire de Physiologie, Faculté de Médecine, France.
Cryobiology. 1994 Dec;31(6):540-8. doi: 10.1006/cryo.1994.1065.
Isolated hepatocytes from the rat were used to assess the maintenance of liver cell function in relation to the composition of the preservation medium. After separation by collagenase, they were incubated in Krebs-Ringer-Bicarbonate medium (KRB), Euro-Collins (EC), or University of Wisconsin (UW) solutions. Potassium influx, cell volume, and transaminase release were measured in cells freshly separated from control livers or from livers preserved in vitro up to 12 h in these media or having undergone orthotopic liver transplantation (OLT). While ion exchange levels were retained in all media, cells shrank significantly in UW but were able to restore their volume after 3 h of liver preservation. With regard to in vivo conditions, UW appears to be the best medium to prevent edema and to maintain more stable potassium exchange and enzyme production. These results are of value for liver transplantation in humans.
从大鼠分离得到的肝细胞被用于评估与保存培养基成分相关的肝细胞功能维持情况。经胶原酶分离后，将它们置于 Krebs - Ringer - 碳酸氢盐培养基（KRB）、欧洲柯林斯液（EC）或威斯康星大学液（UW）中孵育。对从对照肝脏新鲜分离的细胞，或在这些培养基中体外保存长达 12 小时的肝脏细胞，或经历原位肝移植（OLT）的肝脏细胞，测量其钾离子内流、细胞体积和转氨酶释放。虽然在所有培养基中离子交换水平都得以保留，但 UW 中的细胞显著收缩，但在肝脏保存 3 小时后能够恢复其体积。就体内情况而言，UW 似乎是预防水肿以及维持更稳定的钾离子交换和酶产生的最佳培养基。这些结果对人类肝移植具有重要价值。
