Speevak M D, Bérubé N G, McGowan-Jordan I J, Bisson C, Lupton S D, Chevrette M
Department of Biochemistry, Faculty of Medicine, University of Ottawa, Ontario Canada.
Cytogenet Cell Genet. 1995;69(1-2):63-5. doi: 10.1159/000133939.
We have constructed a panel of human x murine microcell hybrids containing individual human chromosomes tagged with a dual selectable marker conferring hygromycin B resistance and ganciclovir sensitivity. Over 500 independent microcell hybrids (B78MC) were generated and more than 200 individually isolated. We have identified the human chromosome content of several B78MC hybrids and verified that the majority are responsive to positive and negative selection. Once fully characterized, this panel will be useful in the study of dominant regulators of gene activity, such as tissue specific regulators and tumor suppressor genes.
我们构建了一组人-鼠微细胞杂种，其中包含携带赋予潮霉素B抗性和更昔洛韦敏感性的双重选择标记的单个人类染色体。产生了500多个独立的微细胞杂种（B78MC），并单独分离出200多个。我们已经鉴定了几种B78MC杂种的人类染色体组成，并证实大多数对正负选择有反应。一旦得到充分表征，该组将有助于研究基因活性的显性调节因子，如组织特异性调节因子和肿瘤抑制基因。
