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鱼类精子发生的起始

The onset of spermatogenesis in fish.

作者信息

Nagahama Y, Miura T, Kobayashi T

机构信息

Department of Developmental Biology, National Institute for Basic Biology, Okazaki.

出版信息

Ciba Found Symp. 1994;182:255-67; discussion 267-70. doi: 10.1002/9780470514573.ch14.

DOI:10.1002/9780470514573.ch14
PMID:7835154
Abstract

Under cultivation conditions, male Japanese eels (Anguilla japonica) have immature testes containing only spermatogonia together with inactive testicular somatic cells, Leydig cells and Sertoli cells. Using a recently developed organ culture system for eel testes, we have shown that hormonal induction of spermatogenesis in eel testes involves gonadotropin stimulation of Leydig cells to produce 11-ketotestosterone, a potent androgen in fish. In turn, 11-ketotestosterone activates Sertoli cells to stimulate premitotic spermatogonia to complete spermatogenesis. Our current research focuses on the isolation and characterization of genes that show altered expression in eel testes during gonadotropin-induced spermatogenesis. One up-regulated and three down-regulated genes have been isolated. Northern blot analysis and in situ hybridization reveal that mRNA for activin B is absent in testes before gonadotropin injection and is abundant in Sertoli cells in testes injected with gonadotropin for one to six days after injection. This stimulation of activin B mRNA is accompanied by spermatogonial proliferation. Gonadotropin treatment also causes a rapid rise in the testicular concentrations of mRNA for 3 beta-hydroxysteroid dehydrogenase, the rate-limiting enzyme for gonadotropin-induced 11-ketotestosterone production. We have also obtained three down-regulated cDNAs which are abundant in testes before gonadotropin treatment and disappear almost completely in testes one day after gonadotropin injection.

摘要

在养殖条件下,雄性日本鳗鲡(Anguilla japonica)的睾丸不成熟,仅含有精原细胞以及不活跃的睾丸体细胞、莱迪希细胞和支持细胞。利用最近开发的鳗鲡睾丸器官培养系统,我们已经表明,鳗鲡睾丸中精子发生的激素诱导涉及促性腺激素刺激莱迪希细胞产生11-酮睾酮,这是鱼类中的一种强效雄激素。反过来,11-酮睾酮激活支持细胞,刺激有丝分裂前的精原细胞完成精子发生。我们目前的研究重点是分离和鉴定在促性腺激素诱导的精子发生过程中鳗鲡睾丸中表达发生变化的基因。已经分离出一个上调基因和三个下调基因。Northern印迹分析和原位杂交显示,在注射促性腺激素之前,睾丸中不存在激活素B的mRNA,而在注射促性腺激素后1至6天的睾丸支持细胞中大量存在。激活素B mRNA的这种刺激伴随着精原细胞的增殖。促性腺激素处理还导致睾丸中3β-羟基类固醇脱氢酶mRNA的浓度迅速升高,3β-羟基类固醇脱氢酶是促性腺激素诱导的11-酮睾酮产生的限速酶。我们还获得了三个下调的cDNA,它们在促性腺激素处理前的睾丸中大量存在,在注射促性腺激素一天后的睾丸中几乎完全消失。

相似文献

1
The onset of spermatogenesis in fish.鱼类精子发生的起始
Ciba Found Symp. 1994;182:255-67; discussion 267-70. doi: 10.1002/9780470514573.ch14.
2
Hormonal induction of all stages of spermatogenesis in vitro in the male Japanese eel (Anguilla japonica).日本雄性鳗鱼(Anguilla japonica)精子发生各阶段的体外激素诱导。
Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5774-8. doi: 10.1073/pnas.88.13.5774.
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Impaired spermatogenesis in the Japanese eel, Anguilla japonica: possibility of the existence of factors that regulate entry of germ cells into meiosis.日本鳗鲡精子发生受损:存在调节生殖细胞进入减数分裂的因子的可能性。
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Two testicular cDNA clones suppressed by gonadotropin stimulation exhibit ZP2- and ZP3-like structures in Japanese eel.在日本鳗鲡中,两个受促性腺激素刺激抑制的睾丸cDNA克隆呈现出类似ZP2和ZP3的结构。
Mol Reprod Dev. 1998 Nov;51(3):235-42. doi: 10.1002/(SICI)1098-2795(199811)51:3<235::AID-MRD2>3.0.CO;2-Q.
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Human chorionic gonadotropin induces all stages of spermatogenesis in vitro in the male Japanese eel (Anguilla japonica).人绒毛膜促性腺激素可在体外诱导雄性日本鳗鲡(Anguilla japonica)精子发生的各个阶段。
Dev Biol. 1991 Jul;146(1):258-62. doi: 10.1016/0012-1606(91)90468-i.
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Comparative studies between in vivo and in vitro spermatogenesis of Japanese eel (Anguilla japonica).日本鳗鲡(Anguilla japonica)体内与体外精子发生的比较研究。
Zoolog Sci. 2002 Mar;19(3):321-9. doi: 10.2108/zsj.19.321.
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Expressional regulation of gonadotropin receptor genes and androgen receptor genes in the eel testis.促性腺激素受体基因和雄激素受体基因在鳗鲡睾丸中的表达调控。
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cDNA cloning of a stage-specific gene expressed during HCG-induced spermatogenesis in the Japanese eel.日本鳗鲡中HCG诱导精子发生过程中表达的阶段特异性基因的cDNA克隆。
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Spermatogenesis-preventing substance in Japanese eel.日本鳗鲡体内的精子发生抑制物质。
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Roles of 11beta-hydroxysteroid dehydrogenase in fish spermatogenesis.11β-羟基类固醇脱氢酶在鱼类精子发生中的作用。
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