Fucci L, Piscopo A, Aniello F, Branno M, Di Gregorio A, Calogero R, Geraci G
Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli Federico II, Italia.
Gene. 1995 Jan 23;152(2):205-8. doi: 10.1016/0378-1119(94)00719-9.
A 2935-bp cDNA clone encoding glutamine synthetase (GS) was isolated from a cDNA library prepared from four-blastomere Paracentrotus lividus sea urchin embryos. The sequence consists of a 75-bp 5' untranslated region (5'-UTR) followed by a 1095-bp coding region corresponding to a 365-amino-acid (aa) protein, a 1747-bp 3'-UTR and a terminal 18-bp poly(A) tail. The encoded protein shows about 66% identical residues, as compared with human and lobster class-II GS. The sequence contains the Mn(2+)-binding aa and the highly conserved aa regions observed in other GS. Northern blot analyses show that the GS mRNA is present in the sea urchin egg and is developmentally regulated in the embryo.
从紫球海胆四细胞期胚胎构建的cDNA文库中分离出一个编码谷氨酰胺合成酶（GS）的2935 bp cDNA克隆。该序列由一个75 bp的5'非翻译区（5'-UTR）、一个对应于365个氨基酸（aa）蛋白质的1095 bp编码区、一个1747 bp的3'-UTR和一个18 bp的末端poly(A)尾组成。与人和龙虾的II类GS相比，编码的蛋白质显示出约66%的相同残基。该序列包含锰离子结合氨基酸以及在其他GS中观察到的高度保守的氨基酸区域。Northern印迹分析表明，GS mRNA存在于海胆卵中，并在胚胎发育过程中受到调控。
