Fucci L, Aniello F, Branno M, Biffali E, Geraci G
Department of Genetics, General and Molecular Biology, University of Naples Federico II, Italy.
Biochim Biophys Acta. 1994 Oct 18;1219(2):539-42. doi: 10.1016/0167-4781(94)90084-1.
A cDNA encoding a new H3 histone variant has been isolated from a Paracentrotus lividus sea urchin embryo cDNA library. The encoded protein is identical to the H3.3 histone subtype identified in other species, with the difference that E replaces D at position 81. The clone corresponds to a transcript of about 1.6 kb, not dependent on DNA replication, present in the unfertilized egg and at all stages of embryonic development. The coding part of the cDNA cross-reacts also with a 0.5 kb H3 late histone mRNA.
已从地中海海胆胚胎cDNA文库中分离出一个编码新H3组蛋白变体的cDNA。所编码的蛋白质与在其他物种中鉴定出的H3.3组蛋白亚型相同，不同之处在于第81位的E取代了D。该克隆对应于一个约1.6 kb的转录本，不依赖于DNA复制，存在于未受精卵和胚胎发育的所有阶段。该cDNA的编码部分也与一个0.5 kb的H3晚期组蛋白mRNA发生交叉反应。
