一种广泛表达的新型C2H2锌指蛋白在小鼠和人类中保守，具有多个共有磷酸化位点。
A widely expressed novel C2H2 zinc-finger protein with multiple consensus phosphorylation sites is conserved in mouse and man.
作者信息
Saotome Y, Winter C G, Hirsh D
机构信息
Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032.
出版信息
Gene. 1995 Jan 23;152(2):233-8. doi: 10.1016/0378-1119(94)00717-7.
We have cloned a murine cDNA whose deduced sequence encodes a 455-amino-acid (aa) zinc-finger protein (Zfp), PZf (penta Zf protein). Sequence analysis shows that PZf has multiple phosphorylation consensus sites for casein kinase II and protein kinase C in its N-terminal portion. This region (aa 1 to 197), which does not share significant homology to known aa sequences, has a number of charged (39 Glu, 15 Asp, 23 Lys, 22 Arg) and hydroxyl (20 Ser, 12 Thr) aa. This potentially phosphorylatable region is followed by five C2H2 Zf in the middle of the protein. The Pzf gene is transcribed as a 5-kb mRNA in all murine tissues examined. The human genome also seems to contain one or more Pzf-related genes.
我们克隆了一个小鼠cDNA，其推导序列编码一个455个氨基酸（aa）的锌指蛋白（Zfp），即PZf（五锌指蛋白）。序列分析表明，PZf在其N端部分具有多个酪蛋白激酶II和蛋白激酶C的磷酸化共有位点。该区域（第1至197个氨基酸）与已知氨基酸序列没有明显同源性，有许多带电荷的氨基酸（39个谷氨酸、15个天冬氨酸、23个赖氨酸、22个精氨酸）和羟基氨基酸（20个丝氨酸、12个苏氨酸）。这个潜在的可磷酸化区域之后是该蛋白中部的五个C2H2锌指。Pzf基因在所有检测的小鼠组织中转录为一个5kb的mRNA。人类基因组似乎也包含一个或多个与Pzf相关的基因。
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