Numoto M, Niwa O, Kaplan J, Wong K K, Merrell K, Kamiya K, Yanagihara K, Calame K
Research Institute for Nuclear Medicine and Biology, Hiroshima University, Japan.
Nucleic Acids Res. 1993 Aug 11;21(16):3767-75. doi: 10.1093/nar/21.16.3767.
We have cloned a cDNA encoding a new murine C2H2 zinc finger protein, ZF5. The 51.3 kD protein contains five GL1-Kruppel type zinc fingers at the C-terminus. At its N-terminus, ZF5 has a 41 amino acid region which was found to be homologous to the N-termini of several other zinc finger proteins. This region defines a new motif within zinc finger proteins which we have named the Zinc finger N-terminal (ZiN) domain. ZF5 binds to two sites in the c-myc promoter and to the -50 bp site of the herpes simplex thymidine kinase promoter. ZF5 is a transcriptional repressor and its repression domain is located N-terminal to the zinc finger domains. A single 4 kb ZF5 mRNA is expressed widely.
我们克隆了一个编码新型小鼠C2H2锌指蛋白ZF5的cDNA。该51.3 kD蛋白在C端含有五个GL1-Kruppel型锌指。在其N端，ZF5有一个41个氨基酸的区域，发现该区域与其他几种锌指蛋白的N端同源。该区域在锌指蛋白中定义了一个新的基序，我们将其命名为锌指N端（ZiN）结构域。ZF5与c-myc启动子中的两个位点以及单纯疱疹胸苷激酶启动子的-50 bp位点结合。ZF5是一种转录抑制因子，其抑制结构域位于锌指结构域的N端。单一的4 kb ZF5 mRNA广泛表达。
