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从嗜盐嗜碱菌中分离和鉴定一种类钙调蛋白

Isolation and characterization of a calmodulin-like protein from Halobacterium salinarium.

作者信息

Rothärmel T, Wagner G

机构信息

Membran- und Bewegungsphysiologie, Botanisches Institut I, Justus Liebig-Universität, Giessen, Germany.

出版信息

J Bacteriol. 1995 Feb;177(3):864-6. doi: 10.1128/jb.177.3.864-866.1995.

DOI:10.1128/jb.177.3.864-866.1995
PMID:7836331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC176675/
Abstract

The first evidence for a calmodulin-like protein in an archaeon, Halobacterium salinarium, is reported here. The calmodulin-like protein, with a molecular mass of 24 kDa and an estimated pI of 4.8, stimulated cyclic nucleotide phosphodiesterase in a calcium-dependent manner. This stimulation could be suppressed by calmodulin inhibitors. The Ca(2+)-binding ability was verified by 45Ca autoradiography.

摘要

本文报道了古细菌盐生盐杆菌中存在类钙调蛋白的首个证据。该类钙调蛋白分子量为24 kDa，估计pI为4.8，以钙依赖方式刺激环核苷酸磷酸二酯酶。这种刺激可被钙调蛋白抑制剂抑制。通过45Ca放射自显影验证了其Ca(2+)结合能力。

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本文引用的文献

1
Sensitivity of Halobacterium salinarium to attractant light stimuli does not change periodically.盐沼盐杆菌对吸引光刺激的敏感性不会周期性变化。
FEBS Lett. 1994 Aug 29;351(1):133-6. doi: 10.1016/0014-5793(94)00844-2.
2
The complete amino acid sequence of the Ca2+-dependent modulator protein (calmodulin) of bovine brain.牛脑钙离子依赖调节蛋白（钙调蛋白）的完整氨基酸序列。
J Biol Chem. 1980 Feb 10;255(3):962-75.
3
Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis.在十二烷基硫酸钠凝胶电泳后，通过硝酸纤维素膜上的45Ca放射自显影法检测钙结合蛋白。
J Biochem. 1984 Feb;95(2):511-9. doi: 10.1093/oxfordjournals.jbchem.a134633.
4
Control of transmembrane ion fluxes to select halorhodopsin-deficient and other energy-transduction mutants of Halobacterium halobium.控制跨膜离子通量以筛选盐生盐杆菌中缺乏嗜盐视紫红质及其他能量转导突变体。
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4308-12. doi: 10.1073/pnas.79.14.4308.
5
Physiological implications of the presence, distribution, and regulation of calmodulin in eukaryotic cells.钙调蛋白在真核细胞中的存在、分布及调节的生理学意义。
Physiol Rev. 1982 Jan;62(1):1-39. doi: 10.1152/physrev.1982.62.1.1.
6
Characterization of the plant nicotinamide adenine dinucleotide kinase activator protein and its identification as calmodulin.植物烟酰胺腺嘌呤二核苷酸激酶激活蛋白的特性及其作为钙调蛋白的鉴定。
Biochemistry. 1980 Jun 24;19(13):3113-20. doi: 10.1021/bi00554a043.
7
Cyclic 3',5'-nucleotide phosphodiesterase. Evidence for and properties of a protein activator.环3',5'-核苷酸磷酸二酯酶。一种蛋白质激活剂的证据及特性
J Biol Chem. 1971 May 10;246(9):2859-69.
8
Transferrin variants in sheep: separation and characterization by polyacrylamide gel electrophoresis and isoelectric focusing.绵羊中的转铁蛋白变体：通过聚丙烯酰胺凝胶电泳和等电聚焦进行分离与表征
Anim Genet. 1986;17(4):343-52. doi: 10.1111/j.1365-2052.1986.tb00727.x.
9
Adenylate cyclase activity in cyanobacteria: activation by Ca(2+)-calmodulin and a calmodulin-like activity.蓝藻中的腺苷酸环化酶活性：由Ca(2+)-钙调蛋白激活及一种类钙调蛋白活性
Biochim Biophys Acta. 1990 Oct 15;1055(1):75-81. doi: 10.1016/0167-4889(90)90093-s.
10
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