Broadley C, Gonzalez D A, Nair R, Koriwchak M J, Ossoff R H, Davidson J M
Department of Otolaryngology, Vanderbilt University Medical Center, Nashville, TN 37232.
Laryngoscope. 1995 Jan;105(1):23-7. doi: 10.1288/00005537-199501000-00008.
A tissue-culture model has been developed for the study of fibroblasts from the canine vocal fold. Laryngeal tissue (lamina propria) obtained from euthanized dogs is rinsed, cut into 1-mm3 pieces, and incubated in 5% carbon dioxide at 37 degrees C. A confluent monolayer is established within several days. Detectable levels of elastin in the tissue culture supernatant are measured by an indirect enzyme-linked immunosorbent assay. Various external agents have been shown to affect elastin production. The effects of KTP laser irradiation, hydrocortisone (1.3 mumol/L), transforming growth factor-beta (10 ng/mL), and human leukocyte elastase have been measured. Thus the canine vocal fold fibroblast tissue culture is established as a model for further investigations to improve wound healing and to understand the wound-healing process following laryngeal microsurgery.
已开发出一种组织培养模型，用于研究犬类声带的成纤维细胞。从安乐死的狗身上获取的喉部组织（固有层）经过冲洗，切成1立方毫米的小块，并在37摄氏度、5%二氧化碳环境中培养。数天内可形成汇合的单层细胞。通过间接酶联免疫吸附测定法测量组织培养上清液中可检测到的弹性蛋白水平。已证明多种外部因素会影响弹性蛋白的产生。已测量了KTP激光照射、氢化可的松（1.3微摩尔/升）、转化生长因子-β（10纳克/毫升）和人白细胞弹性蛋白酶的作用。因此，犬类声带成纤维细胞组织培养已被确立为一个模型，用于进一步研究以改善伤口愈合，并了解喉显微手术后的伤口愈合过程。
