Branski Ryan C, Rosen Clark A, Verdolini Katherine, Hebda Patricia A
Department of Communication Science and Disorders, University of Pittsburgh, Pittsburgh, PA, USA.
J Voice. 2005 Jun;19(2):283-9. doi: 10.1016/j.jvoice.2004.04.003.
This study seeks to determine the ability of enzyme-linked immunosorbent assays (ELISAs) of vocal fold secretions to detect and describe the acute tissue response to injury in a rabbit vocal fold model. Vocal fold secretions were collected before the induction of a unilateral surgical injury to the vocal fold and at 6 timepoints after injury (1, 5, 7, 10, 14, and 21 days). Secretions were then subjected to ELISAs to assess concentrations of interleukin-1 beta (IL-1beta) and prostaglandin-E2 (PGE-2). The results indicate that ELISAs may be useful in documenting fluctuations in these markers associated with the wound healing process in the rabbit model. The temporal expression of both IL-1beta and PGE-2 was consistent with their proposed roles in the wound healing cascade in other systems, pointing to the potential that surface secretions may be at least partial indicators of wound healing events within the tissue.
本研究旨在确定在兔声带模型中，通过声带分泌物的酶联免疫吸附测定（ELISA）来检测和描述急性组织损伤反应的能力。在单侧手术损伤声带之前以及损伤后的6个时间点（1、5、7、10、14和21天）收集声带分泌物。然后对分泌物进行ELISA，以评估白细胞介素-1β（IL-1β）和前列腺素-E2（PGE-2）的浓度。结果表明，ELISA可能有助于记录兔模型中与伤口愈合过程相关的这些标志物的波动情况。IL-1β和PGE-2的时间表达与它们在其他系统伤口愈合级联反应中所起的作用一致，表明表面分泌物可能至少部分反映了组织内伤口愈合事件的可能性。
