Müller A, Mayberry W, Acuff R, Thedford S, Browder W, Williams D
Department of Surgery, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614.
Microbios. 1994;79(321):253-61.
The preparation of pharmaceutical grade (1-->3)-beta-D-glucans from Saccharomyces cerevisiae requires that the microparticulate glucans which are employed as the starting material for drug production be of the highest purity. Potential contaminants of the (1-->3)-beta-D-glucan isolation process are yeast cell wall lipids, which are frequently found in association with glucans. The lipid content of yeast-derived (1-->3)-beta-D-glucan during various stages of the isolation process by methyl esterification and total ion gas chromatography and mass spectrometry (GC-MS) was examined. Following sequential alkaline and acid hydrolyses, the total lipid content of (1-->3)-beta-D-glucan was 100.4 nmol/mg with 9-cis-C 18:1 fatty acid accounting for 45.3 nmol/mg glucan (45%). Following ethanol extraction of the (1-->3)-beta-D-glucan, lipid was not detected. Indeed, fatty acid methyl esters were not detectable by total ion GC-MS in all twelve samples investigated. These data demonstrate that the isolation process for yeast-derived (1-->3)-beta-D-glucan effectively reduces contamination by yeast cell wall lipids.
从酿酒酵母制备药用级（1→3）-β-D-葡聚糖要求用作药物生产起始原料的微粒状葡聚糖具有最高纯度。（1→3）-β-D-葡聚糖分离过程中的潜在污染物是酵母细胞壁脂质，它们经常与葡聚糖结合存在。通过甲酯化以及全离子气相色谱和质谱法（GC-MS）检测了酵母来源的（1→3）-β-D-葡聚糖在分离过程各个阶段的脂质含量。经过连续的碱水解和酸水解后，（1→3）-β-D-葡聚糖的总脂质含量为100.4 nmol/mg，其中9-顺式-C 18:1脂肪酸占45.3 nmol/mg葡聚糖（45%）。用乙醇萃取（1→3）-β-D-葡聚糖后，未检测到脂质。实际上，在所研究的全部12个样品中，通过全离子GC-MS均未检测到脂肪酸甲酯。这些数据表明，酵母来源的（1→3）-β-D-葡聚糖的分离过程有效地减少了酵母细胞壁脂质的污染。
