Satterwhite T K, Evans D G, DuPont H L, Evans D J
Lancet. 1978 Jul 22;2(8082):181-4. doi: 10.1016/s0140-6736(78)91921-9.
The role of colonisation factor antigen (C.F.A.) in diarrhoea was studied in volunteers who ingested either E. coli H-10407, which possessed C.F.A., or H-10407-P, which lacked C.F.A. In the first study the dose given was 10(6) C.F.A.+ or C.F.A.- E. coli; in the second study the dose was 10(8) of the same organisms. Diarrhoea, with or without other symptoms, occurred only in those who ingested 10(8) C.F.A.+ E. coli. Those volunteers taking the C.F.A.- strain shed the test organism in the stool for only 2 or 3 days whereas those ingesting the C.F.A+ strain shed the organism until the end of the study (7 days). Antibody responses in those ingesting the C.F.A.+ strain were: anti-C.F.A., 7 of 12 volunteers; antitoxin, 6 of 13; and anti-O78 somatic antigen, 11 of 13. No volunteer ingesting the C.F.A. organism seroconverted to any of the antigens. The study showed the importance of C.F.A. in the pathogenesis of human disease due to toxigenic E. coli.
在摄入携带定居因子抗原（C.F.A.）的大肠杆菌H-10407或缺乏C.F.A.的H-10407-P的志愿者中，研究了定居因子抗原（C.F.A.）在腹泻中的作用。在第一项研究中，给予的剂量是10⁶个携带或不携带C.F.A.的大肠杆菌；在第二项研究中，剂量是10⁸个相同的生物体。腹泻，无论有无其他症状，仅发生在摄入10⁸个携带C.F.A.的大肠杆菌的志愿者中。那些服用不携带C.F.A.菌株的志愿者在粪便中排出受试生物体的时间仅为2或3天，而那些摄入携带C.F.A.菌株的志愿者在研究结束（7天）前一直排出该生物体。摄入携带C.F.A.菌株的志愿者的抗体反应为：抗C.F.A.，12名志愿者中有7名；抗毒素，13名中有6名；抗O78菌体抗原，13名中有11名。没有摄入携带C.F.A.生物体的志愿者血清转化为任何一种抗原。该研究表明C.F.A.在产毒性大肠杆菌引起的人类疾病发病机制中的重要性。
