Newsome P M, Burgess M N, Burgess M R, Coney K A, Goddard M E, Morris J A
J Med Microbiol. 1987 Feb;23(1):19-28. doi: 10.1099/00222615-23-1-19.
Oral inoculation of neonatal MFI mice with enterotoxigenic strains of Escherichia coli that possessed the K99 or F41 antigen or both resulted in severe diarrhoea with high mortality. The diarrhoea was associated with increased fluid in the gut, greatly increased numbers of E. coli in gut homogenates and reduced weight gain compared to control animals. Further studies with strain B44 demonstrated greatly increased numbers of E. coli on the surface of the intestinal mucosa and haemo-concentration. The infection was transmissible between litter-mates. There was no evidence of invasion of the intestinal tissue of infected animals. Gnotobiotic Balb C mice and endotoxin-resistant mice were susceptible to oral inoculation with bovine enterotoxigenic E. coli strains, but neonatal rats were not susceptible to infection with enterotoxigenic E. coli strains B44 or 431. Porcine strains of E. coli that possessed K88 or 987P antigen did not infect neonatal MFI mice but an "atypical" porcine strain (431) which possessed both K99 and F41 antigens caused diarrhoea and a high mortality. The disease in neonatal mice resembled acute diarrhoea caused by these bacteria in other species, particularly the calf, and the model should be of value in assessing the efficacy of therapeutic agents.
用携带K99或F41抗原或两者皆有的产肠毒素大肠杆菌菌株经口接种新生MFI小鼠，会导致严重腹泻并伴有高死亡率。与对照动物相比，腹泻与肠道内液体增多、肠道匀浆中产肠毒素大肠杆菌数量大幅增加以及体重增加减少有关。对B44菌株的进一步研究表明，肠黏膜表面的产肠毒素大肠杆菌数量大幅增加且出现血液浓缩。这种感染可在同窝幼崽之间传播。没有证据表明感染动物的肠道组织受到侵袭。无菌Balb C小鼠和内毒素抗性小鼠对口服接种牛产肠毒素大肠杆菌菌株敏感，但新生大鼠对产肠毒素大肠杆菌菌株B44或431感染不敏感。携带K88或987P抗原的猪源大肠杆菌菌株不会感染新生MFI小鼠，但一株同时携带K99和F41抗原的“非典型”猪源菌株（431）会导致腹泻和高死亡率。新生小鼠的这种疾病类似于这些细菌在其他物种（尤其是犊牛）中引起的急性腹泻，该模型在评估治疗药物的疗效方面应具有价值。
