Overgaard Larsen J, Tandrup T, Braendgaard H
Stereological Research Laboratory, University of Aarhus, Denmark.
Acta Neuropathol. 1994;88(4):307-12. doi: 10.1007/BF00310374.
The effects of acrylamide intoxication on the numbers of granule and Purkinje cells and the volume of Purkinje cell perikarya have been evaluated with stereological methods. The analysis was carried out in the cerebella of rats that had received a dose of 33.3 mg/kg acrylamide, twice a week, for 7.5 weeks. The total numbers of cerebellar granule and Purkinje cells were estimated using the optical fractionator and the mean volume of the Purkinje cell perikarya was estimated with the vertical rotator technique. The volumes of the molecular layer, the granular cell layer and the white matter were estimated using the Cavalieri principle. The mean weight of the cerebellum of the intoxicated rats was 7% lower than that of the control rats (2P = 0.001). The numbers of the Purkinje cells and granule cells were the same in both groups, but the mean volume of the perikarya of the Purkinje cells in the intoxicated rats was 10.5% less than that of the control group (2P = 0.004). The volume of the granular cell layer was reduced by 15% (2P = 0.006) but there were no differences in the volumes of the molecular layer and the white matter in the intoxicated and control animals.
已采用体视学方法评估了丙烯酰胺中毒对颗粒细胞和浦肯野细胞数量以及浦肯野细胞胞体体积的影响。分析在每周两次接受33.3mg/kg丙烯酰胺剂量、持续7.5周的大鼠小脑中进行。使用光学分割器估计小脑颗粒细胞和浦肯野细胞的总数，并用垂直旋转器技术估计浦肯野细胞胞体的平均体积。使用卡瓦列里原理估计分子层、颗粒细胞层和白质的体积。中毒大鼠小脑的平均重量比对照大鼠低7%（P=0.001）。两组的浦肯野细胞和颗粒细胞数量相同，但中毒大鼠浦肯野细胞胞体的平均体积比对照组小10.5%（P=0.004）。颗粒细胞层的体积减少了15%（P=0.006），但中毒动物和对照动物的分子层和白质体积没有差异。
