Hemmer J, Kreidler J, Kraft K, Schön E
University of Ulm, Division of Tumor Biology, Germany.
Anticancer Res. 1994 Sep-Oct;14(5B):2077-83.
Thirty-one patients with squamous cell carcinomas of the oral cavity who underwent intra-arterial induction chemotherapy with 5-fluorouracil and cisplatin were analysed by DNA flow cytometry. Seven tumors consisted exclusively of diploid cells and 24 were characterized by the presence of an aneuploid tumor cell line. Response to chemotherapy as well as survival were significantly better in the diploid group. The cytotoxic effect of the treatment on aneuploid tumor cell populations was monitored in vivo by serial DNA flow cytometric measurements. Decreasing numbers of aneuploid cells were detected in all but two cases (92%); twelve (50%) showed a complete disappearance of the aberrant cell line at the end of chemotherapy. All non-responders showed persisting aneuploid tumor cells at the end of treatment. Even if response to chemotherapy was better in the group with a complete disappearance of the aneuploid cell line in the course of treatment, the survival outcome of these patients did not differ significantly from those who showed persistence of aneuploid tumor cells.
对31例接受5-氟尿嘧啶和顺铂动脉内诱导化疗的口腔鳞状细胞癌患者进行了DNA流式细胞术分析。7个肿瘤仅由二倍体细胞组成，24个肿瘤的特征是存在非整倍体肿瘤细胞系。二倍体组对化疗的反应以及生存率明显更好。通过连续的DNA流式细胞术测量在体内监测治疗对非整倍体肿瘤细胞群体的细胞毒性作用。除两例外（92%），所有病例中检测到的非整倍体细胞数量均减少；12例（50%）在化疗结束时异常细胞系完全消失。所有无反应者在治疗结束时均显示非整倍体肿瘤细胞持续存在。即使在治疗过程中非整倍体细胞系完全消失的组对化疗的反应更好，但这些患者的生存结果与显示非整倍体肿瘤细胞持续存在的患者没有显著差异。
