Engelman A
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-0560.
Bioessays. 1994 Nov;16(11):797-9. doi: 10.1002/bies.950161105.
Although retroviral integration requires specific viral DNA sequences, factors which govern the choice of a chromosomal target site within an infected cell are less clear. For example, certain chromosomal regions may be inaccessible to the viral integration machinery, while others may favor integration. A recent paper by Withers-Ward et al.(1) addresses this issue using a polymerase chain reaction-based assay capable of identifying single integration events within a large population of infected cells. Their results show that integration can occur into many different chromosomal regions, and that local DNA structure can influence the site of integration within a given region.
尽管逆转录病毒整合需要特定的病毒DNA序列，但在受感染细胞内决定染色体靶位点选择的因素却不太清楚。例如，某些染色体区域可能无法被病毒整合机制所触及，而其他区域可能有利于整合。Withers-Ward等人近期发表的一篇论文(1)使用了一种基于聚合酶链反应的检测方法来解决这个问题，该方法能够识别大量受感染细胞中的单个整合事件。他们的结果表明，整合可以发生在许多不同的染色体区域，并且局部DNA结构可以影响给定区域内的整合位点。
