Molecular Microbiology and Structural Biochemistry (MMSB), CNRS, University of Lyon 1, UMR 5086, 69367 Lyon, France.
Viral DNA Integration and Chromatin Dynamics Network (DyNAVir), 33076 Bordeaux, France.
Viruses. 2022 Jun 27;14(7):1397. doi: 10.3390/v14071397.
Retroviral integrase is a multimeric enzyme that catalyzes the integration of reverse-transcribed viral DNA into the cellular genome. Beyond integration, the Human immunodeficiency virus type 1 (HIV-1) integrase is also involved in many other steps of the viral life cycle, such as reverse transcription, nuclear import, virion morphogenesis and proviral transcription. All these additional functions seem to depend on the action of the integrase C-terminal domain (CTD) that works as a molecular hub, interacting with many different viral and cellular partners. In this review, we discuss structural issues concerning the CTD, with particular attention paid to its interaction with nucleic acids. We also provide a detailed map of post-translational modifications and interaction with molecular partners.
逆转录病毒整合酶是一种多聚体酶，可催化逆转录病毒 DNA 整合到细胞基因组中。除了整合作用外，人类免疫缺陷病毒 1 型（HIV-1）整合酶还参与病毒生命周期的许多其他步骤，如逆转录、核输入、病毒形态发生和前病毒转录。所有这些额外的功能似乎都依赖于整合酶 C 末端结构域（CTD）的作用，该结构域作为分子枢纽，与许多不同的病毒和细胞伙伴相互作用。在这篇综述中，我们讨论了 CTD 的结构问题，特别关注其与核酸的相互作用。我们还提供了一个详细的翻译后修饰图谱和与分子伙伴的相互作用图。
