Mentzel S, de Leeuw E P, van Son J P, Dijkman H B, de Jong A S, Koene R A, Assmann K J
Department of Pathology, University Hospital Nijmegen, The Netherlands.
Biol Chem Hoppe Seyler. 1994 Sep;375(9):623-7. doi: 10.1515/bchm3.1994.375.9.623.
SDS-PAGE of affinity-purified APA under reducing conditions showed in addition to the specific APA band of M(r) 130 kD, a second band of M(r) 43 kD. Internal amino acid sequencing of three tryptic peptides from this second band, that was cut out of the polyacrylamide gel, matched the actin sequence. The identity of the 43 kD band was also confirmed by Western blotting.
在还原条件下对亲和纯化的抗胰蛋白酶进行的SDS-PAGE分析显示，除了130 kD的特异性抗胰蛋白酶条带外，还有一条43 kD的条带。从聚丙烯酰胺凝胶上切下的该第二条带的三个胰蛋白酶肽段的内部氨基酸测序与肌动蛋白序列匹配。43 kD条带的身份也通过蛋白质免疫印迹得到了证实。
