Jurman M E, Boland L M, Liu Y, Yellen G
Massachusetts General Hospital, Boston.
Biotechniques. 1994 Nov;17(5):876-81.
Electrophysiological study of transiently transfected cells requires the identification of individual cells that express the protein of interest. We describe a simple, quick and inexpensive method for visually identifying cells that have been co-transfected with an expression plasmid for a lymphocyte surface antigen (CD8-alpha). Transfected cells are incubated briefly with polystyrene microspheres (4.5 microns diameter) that have been precoated with antibody to CD8. Cells expressing CD8 on their surface are decorated with many beads and are thus readily distinguishable from untransfected cells. Beads already coated with antibody are available commercially. The method takes less than five minutes and requires no reagent preparation or special equipment for visualization of the beads.
对瞬时转染细胞进行电生理研究需要识别表达感兴趣蛋白质的单个细胞。我们描述了一种简单、快速且廉价的方法，用于直观地识别已与淋巴细胞表面抗原（CD8-α）表达质粒共转染的细胞。将转染细胞与预先包被有抗CD8抗体的聚苯乙烯微球（直径4.5微米）短暂孵育。表面表达CD8的细胞会被许多珠子标记，因此很容易与未转染细胞区分开来。已包被抗体的珠子有商业产品可供使用。该方法耗时不到五分钟，无需试剂制备或特殊设备来观察珠子。
