Peterka M, Likovský Z, Peterková R
Institute of Experimental Medicine, Academy of Sciences, Czech Republic, Prague.
Physiol Res. 1994;43(4):223-8.
The proportion of proliferating erythroblasts, i.e. proerythroblasts, basophilic erythroblasts and polychromatophilic erythroblasts in blood islands of the chick embryo yolk sac, were counted during embryonic days 2-10. From day 2 when high amounts of erythroblasts signalized the onset of embryonic erythropoiesis, the percentage of less mature erythroid cells gradually decreased. Intraamniotic injection of cyclosporin A in doses 1.5 or 15.0 micrograms per embryo on day 5 led to significant changes in the proportion of proliferating erythroblasts in the yolk sac blood islands. We speculate that these changes were caused initially by the release of the more mature cells into the circulation and later by a dose-dependent decrease in the number of stem cells. The estimation of proerythroblast percentage from all proliferating erythroblasts in the yolk sac blood islands may serve as a valuable indication of toxic damage in the late avian embryo.
在鸡胚卵黄囊血岛中，对增殖性成红细胞（即原红细胞、嗜碱性成红细胞和多染性成红细胞）的比例进行了胚胎第2至10天的计数。从第2天开始，大量成红细胞表明胚胎红细胞生成开始，较不成熟的红细胞类细胞百分比逐渐下降。在第5天给每个胚胎腹腔内注射1.5或15.0微克剂量的环孢素A，导致卵黄囊血岛中增殖性成红细胞的比例发生显著变化。我们推测，这些变化最初是由更成熟的细胞释放到循环中引起的，随后是干细胞数量的剂量依赖性减少。从卵黄囊血岛中所有增殖性成红细胞中估算原红细胞百分比，可能是晚期禽类胚胎中毒性损伤的一个有价值指标。
