Likovský Z, Peterka M, Peterková R
Institute of Experimental Medicine, Czech Academy of Sciences, Prague.
Funct Dev Morphol. 1993;3(1):3-9.
The nucleolar test was used for evaluation of the rRNA biosynthesis in two populations of chick embryonic cells. In chick embryos, practically 100 per cent of proerythroblasts (starting from embryonic day 3) as well as hepatocytes (starting with embryonic day 5) synthetized nucleolar, i.e. ribosomal RNA. In consequence of a single administration of the well known teratogens--actinomycin D, cyclophosphamide or hydrocortisone--the percentage of rRNA synthetizing proerythroblasts and hepatocytes decreased characteristically--in dependence on the teratogenic drug. The presented method can be employed when toxicity of drugs or other chemical compounds is evaluated in cell populations possessing high metabolic and proliferative activity.
核仁试验用于评估鸡胚细胞两个群体中的rRNA生物合成。在鸡胚中，实际上100%的原红细胞（从胚胎第3天开始）以及肝细胞（从胚胎第5天开始）合成核仁，即核糖体RNA。单次给予众所周知的致畸剂——放线菌素D、环磷酰胺或氢化可的松后，合成rRNA的原红细胞和肝细胞的百分比会依致畸药物的不同而呈现特征性下降。当评估具有高代谢和增殖活性的细胞群体中药物或其他化合物的毒性时，可以采用本文提出的方法。
