Sanchez T, Vanderkolk J, Seay S, Heifets L
National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
Tuber Lung Dis. 1994 Oct;75(5):386-90. doi: 10.1016/0962-8479(94)90087-6.
To determine the effect of a delay in processing blood specimens, that may occur by mailing them to a specialized laboratory, on the quantitation of the number of mycobacteria.
Simulated blood specimens in sodium polyanetholium sulfonate (SPS)-containing vacutainers were spiked with Mycobacterium avium, and stored for 4 days after addition of clarithromycin or rifabutin, or remained drug-free. Daily samples were lysed with sodium desoxycholate, and inoculated onto 7H11 agar plates for the subsequent colony-forming units (CFU)/ml count. The isolation rates on two media, 7H11 agar and BACTEC 12B broth, were determined with 1212 specimens from AIDS patients.
The number of bacteria in blood specimens remained without substantial change during the period of storage, even in the presence of clarithromycin or rifabutin, especially after refrigeration for 2 or 24 h before storage. 7H11 agar and 12B broth were equally sensitive for isolation of M. avium.
A 4-day delay in processing blood specimens in SPS vacutainers does not affect the results of quantitation of the number of mycobacteria. Combined use of 12B broth and 7H11 agar increased the overall recovery along with an opportunity to quantitate the number of CFU/ml.
确定将血液标本邮寄至专业实验室可能导致的处理延迟对分枝杆菌数量定量的影响。
将含有鸟分枝杆菌的模拟血液标本加入含聚茴香脑磺酸钠（SPS）的真空采血管中，在添加克拉霉素或利福布汀后储存4天，或不添加药物。每日样本用脱氧胆酸钠裂解，并接种到7H11琼脂平板上，随后进行菌落形成单位（CFU）/毫升计数。用1212份艾滋病患者的标本测定了7H11琼脂和BACTEC 12B肉汤两种培养基的分离率。
即使存在克拉霉素或利福布汀，血液标本中的细菌数量在储存期间仍无显著变化，尤其是在储存前冷藏2或24小时后。7H11琼脂和12B肉汤对鸟分枝杆菌的分离同样敏感。
在含SPS的真空采血管中处理血液标本延迟4天不影响分枝杆菌数量定量结果。联合使用12B肉汤和7H11琼脂可提高总体回收率，并提供定量CFU/毫升数量的机会。
