Fiorentini D, Cipollone M, Galli M C, Pugnaloni A, Biagini G, Landi L
Dipartimento di Biochimica G. Moruzzi, Università di Bologna, Italy.
Free Radic Res. 1994 Oct;21(5):329-39. doi: 10.3109/10715769409056585.
The aim of this work was to characterize large unilamellar vesicles (LUVETs) prepared by a hand-driven extrusion device in order to use them for studies of lipid peroxidation and antioxidant activity. Vesicle structure and size were examined by electron microscopy. Lipid and antioxidant content was determined before and after the extrusion procedure. Then LUVETs were subjected to autoxidation initiated by both the lipid-soluble 2,2'-azobis(2,4-dimethylvaleronitrile) and the water-soluble 2,2'-azobis(2-amidinopropane hydrochloride) azocompounds. The results demonstrated that: i) LUVETs prepared with lipid concentrations ranging between 25 and 150 mM were essentially unilamellar and reasonably homogeneous, with an average diameter of 90 nm; ii) the phospholipid, cholesterol and antioxidant amounts retained by filters were about 10-15%; iii) LUVETs were suitable for autoxidation studies initiated by the water-soluble azocompound both in the absence and presence of antioxidants. The lipid-soluble azocompound could be used only at low concentrations and its vesicle content had to be determined since part of the initiator was not incorporated into the lipid bilayer. These data suggest that LUVETs seem to be recommended for studies of lipid peroxidation and antioxidant activity.
这项工作的目的是对通过手动挤压装置制备的大单层囊泡（LUVETs）进行表征，以便将其用于脂质过氧化和抗氧化活性的研究。通过电子显微镜检查囊泡的结构和大小。在挤压过程前后测定脂质和抗氧化剂含量。然后，LUVETs分别受到脂溶性的2,2'-偶氮二（2,4-二甲基戊腈）和水溶性的2,2'-偶氮二（2-脒基丙烷盐酸盐）偶氮化合物引发的自氧化作用。结果表明：i）用浓度在25至150 mM之间的脂质制备的LUVETs基本上是单层的且相当均匀，平均直径为90 nm；ii）过滤器保留的磷脂、胆固醇和抗氧化剂的量约为10 - 15%；iii）无论是在不存在还是存在抗氧化剂的情况下，LUVETs都适用于由水溶性偶氮化合物引发的自氧化研究。脂溶性偶氮化合物只能在低浓度下使用，并且由于部分引发剂未掺入脂质双层中，因此必须测定其囊泡含量。这些数据表明，LUVETs似乎推荐用于脂质过氧化和抗氧化活性的研究。
