Minassian C, Mithieux G
Institut National de la Santé et de la Recherche Médicale, Unit 197, Faculty of Medicine Alexis Carrel, Lyon, France.
Comp Biochem Physiol B Biochem Mol Biol. 1994 Sep;109(1):99-104. doi: 10.1016/0305-0491(94)90146-5.
We have studied the time course of hepatic and renal microsomal glucose-6 phosphatase (Glc-6Pase) during long-term fasting in the rat. Liver microsomal Glc-6Pase increases up to 48 hr and significantly decreases after 48 hr of fasting. The following activities were determined at 0, 24, 48, 72 and 96 hr: 0.31 +/- 0.02; 0.50 +/- 0.02; 0.54 +/- 0.03; 0.44 +/- 0.03; 0.44 +/- 0.01 mumol min-1 mg protein-1, respectively (all values are means +/- SEM, n = 6). Concomitantly, kidney microsomal Glc-6Pase progressively increases throughout the fast (Vm = 0.21 +/- 0.01; 0.26 +/- 0.004; 0.30 +/- 0.01; 0.37 +/- 0.02; 0.40 +/- 0.01 mumol min-1 mg protein-1, from 0 to 96 hr, respectively). These data suggest that the differential expression of Glc-6Pase activity in the liver and the kidney during long-term fasting could have an important role in the shift from a principally hepatic gluconeogenesis to a hepatic and renal gluconeogenesis.
我们研究了大鼠长期禁食期间肝脏和肾脏微粒体葡萄糖-6磷酸酶（Glc-6Pase）的时间变化过程。肝脏微粒体Glc-6Pase在禁食48小时内升高，禁食48小时后显著下降。在0、24、48、72和96小时测定了以下活性：分别为0.31±0.02；0.50±0.02；0.54±0.03；0.44±0.03；0.44±0.01 μmol min-1 mg蛋白-1（所有数值均为平均值±标准误，n = 6）。同时，肾脏微粒体Glc-6Pase在整个禁食过程中逐渐升高（从0到96小时，Vm分别为0.21±0.01；0.26±0.004；0.30±0.01；0.37±0.02；0.40±0.01 μmol min-1 mg蛋白-1）。这些数据表明，长期禁食期间肝脏和肾脏中Glc-6Pase活性的差异表达可能在从主要的肝脏糖异生向肝脏和肾脏糖异生的转变中起重要作用。
