Minassian C, Zitoun C, Mithieux G
INSERM U. 197, Faculté de Médecine Alexis Carrel, Lyon, France.
Mol Cell Biochem. 1996 Feb 9;155(1):37-41. doi: 10.1007/BF00714331.
We have studied the role of Glc6Pase mRNA abundance in the time course of Glc6Pase activity in liver and kidney during long-term fasting in rat. Refered to the mRNA level in the fed state, Glc6Pase mRNA abundance was increased by 3.5 +/- 0.5 and 3.7 +/- 0.5 times (mean +/- S.E.M., n = 5) in the 24 h and 48 h-fasted liver, respectively. Then, the liver Glc6Pase mRNA was decreased to the level of the fed liver after 72 and 96 h of fasting (1.0 +/- 0.3 and 1.4 +/- 0.3). In the kidney, Glc6Pase mRNA abundance was increased by 2.7 +/- 1.0 and 5 +/- 1.2 times at 24 and 48 h of fasting, respectively. Then, it plateaued at the level of the 48 h fasted kidney after 72 h and 96 h of fasting (4.5 +/- 1.0 and 4.3 +/- 1.0). After 24 and 48 h-refeeding, the abundance of Glc6Pase mRNA in 48 h-fasted rats was decreased to the level found in the liver and kidney of fed rats. The time course of the activity of Glc6Pase catalytic subunit during fasting and refeeding was strikingly parallel to the time course of Glc6Pase mRNA level in respective tissues. These data strongly suggest that the differential expression of Glc6Pase activity in liver and kidney in the course of fasting may be accounted for by the respective time course of mRNA abundance in both organs.
我们研究了在大鼠长期禁食过程中，葡萄糖6磷酸酶（Glc6Pase）mRNA丰度在肝脏和肾脏中Glc6Pase活性随时间变化过程中的作用。与进食状态下的mRNA水平相比，禁食24小时和48小时的肝脏中，Glc6Pase mRNA丰度分别增加了3.5±0.5倍和3.7±0.5倍（平均值±标准误，n = 5）。然后，禁食72小时和96小时后，肝脏Glc6Pase mRNA降至进食肝脏的水平（1.0±0.3和1.4±0.3）。在肾脏中，禁食24小时和48小时时，Glc6Pase mRNA丰度分别增加了2.7±1.0倍和5±1.2倍。然后，在禁食72小时和96小时后，其稳定在禁食48小时肾脏的水平（4.5±1.0和4.3±1.0）。在禁食48小时的大鼠重新进食24小时和48小时后，Glc6Pase mRNA丰度降至进食大鼠肝脏和肾脏中的水平。禁食和重新进食期间Glc6Pase催化亚基活性的时间变化过程与各组织中Glc6Pase mRNA水平的时间变化过程显著平行。这些数据强烈表明，禁食过程中肝脏和肾脏中Glc6Pase活性的差异表达可能是由两个器官中mRNA丰度各自的时间变化过程所导致的。
