Eweda W, Lunau S, Fortnagel P
Universität Hamburg, Abteilung Mikrobiologie, Germany.
Microbiol Res. 1994 Nov;149(4):331-6. doi: 10.1016/S0944-5013(11)80080-7.
A promoter-monocistronic structural gene complex from genomic DNA of Bacillus megaterium has been isolated and sequenced. The activity of the promoter during sporulation was measured in B. subtilis using a fusion with the xylE gene of Pseudomonas putida which codes for a catechol-2,3-dioxygenase. From the time of activation in sporulating cells and the activity in a set of defined B. subtilis sporulation mutants we conclude that the promoter requires an active sigmaF-factor of RNA-polymerase. Since this sigma-factor is active only in forespores and not in the mothercell compartment it is likely that we have identified a forespore specific gene of B. megaterium. Its function is still unknown.
已从巨大芽孢杆菌的基因组DNA中分离并测序了一个启动子-单顺反子结构基因复合体。利用与恶臭假单胞菌的xylE基因融合的方法，在枯草芽孢杆菌中测定了该启动子在芽孢形成过程中的活性，xylE基因编码儿茶酚-2,3-双加氧酶。从芽孢形成细胞中的激活时间以及一组确定的枯草芽孢杆菌芽孢形成突变体中的活性，我们得出结论，该启动子需要RNA聚合酶的活性σF因子。由于这种σ因子仅在前芽孢中具有活性，而在母细胞区室中无活性，因此我们很可能已经鉴定出了巨大芽孢杆菌的一个前芽孢特异性基因。其功能仍然未知。
