Kubota Y, Koga T, Imayama S, Hori Y
Department of Dermatology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Contact Dermatitis. 1994 Oct;31(4):217-9. doi: 10.1111/j.1600-0536.1994.tb01991.x.
To investigate possible involvement of Th1-type immunoreaction in the development of skin lesions of atopic dermatitis (AD), we measured mite-antigen-stimulated production of IL-2, IL-4 and IFN-gamma, using peripheral blood mononuclear cells (PBMC) obtained from 23 patients with AD who developed positive patch test reactions to house dust mite antigens extracted from Dermatophagoides pteronyssinus (Dp). Incubation of these PBMC with the Dp antigen for 72 h produced marked secretion of IL-2 and IFN-gamma, but not IL-4, indicating that Dp-specific T cells were found in these patients' circulating peripheral blood and were capable of producing IL-2 and IFN-gamma, known as major mediators of the delayed-type allergic reaction. Our study suggests that Th1-type cells may be involved in the development of skin lesions of AD patients with positive patch test reactions to house dust mite antigen.
为了研究Th1型免疫反应在特应性皮炎（AD）皮肤病变发展中可能的作用，我们使用从23例对从粉尘螨（Dp）中提取的屋尘螨抗原斑贴试验呈阳性反应的AD患者获得的外周血单个核细胞（PBMC），测量了螨抗原刺激的IL-2、IL-4和IFN-γ的产生。将这些PBMC与Dp抗原孵育72小时后，产生了显著的IL-2和IFN-γ分泌，但没有IL-4分泌，这表明在这些患者的循环外周血中发现了Dp特异性T细胞，并且它们能够产生IL-2和IFN-γ，这两种物质是迟发型过敏反应的主要介质。我们的研究表明，Th1型细胞可能参与了对屋尘螨抗原斑贴试验呈阳性反应的AD患者皮肤病变的发展。
