[Laser Raman spectrometry study on experimental galactose-induced cataract].

In order to observe the dynamic changes of hydration in galactose induced cataract, laser Raman spectrometry was used to study the water-content of lens nuclear of Wistar rats after fed them on galactose by 3, 7, 9, 11 and 17 days. The results showed that, (1) during the formation of cataract, the ratio of I3390/I2935 increased from 0.31 (3 days) to 2.26 (17 days). The water content in the nuclear was significantly increased. (2) the hydration of lens nuclear could be divided into two phases. The ratio of I3390/I2935 was increased slowly and steadily by 11 days after galactose feeding. Then, the ratio turned to increase quite fast till 17 days. It indicates that the hydration of nuclear is changed simultaneously with the formation of cataract.