Constantopoulos A, Papadaki-Papandreou U, Papaconstantinou E
Department of Pediatrics, Athens University, Aghia Sophia Children's Hospital, Greece.
Experientia. 1995 Jan 15;51(1):16-8.
The levels of beta-endorphin and Leu-enkephalin were measured in the plasma of 23 children (16 male, 7 female) who were admitted to the Children's Hospital for elective (non-emergency) surgery, adenoidectomy and/or tonsillectomy. Two blood samples were obtained preoperatively. The first sample was taken during admission to the hospital; the second on 24 h later and just 5 min before the operation, without pre-medication or narcosis. beta-endorphin and Leu-enkephalin levels were measured using a RIA method after solid phase extraction of the samples. Mean beta-endorphin concentrations during admission to the hospital were 11.77 +/- 9.36 pg/ml. Twenty four hours later, and just 5 min before surgery, the mean levels of beta-endorphin were increased to 27.45 +/- 16.66 pg/ml. A statistically significant increase (t-value = -5.42) was found for beta-endorphin levels 5 min before the operation compared with 24 h earlier (2-tail probability p < 0.0001). The Wilcoxon matched-pairs signed-ranks-test also showed p < 0.0001 (Z = -4.197). In contrast, Leu-enkephalin levels remained unchanged. The mean values were 11.44 +/- 1.49 pg/ml on the day of admission, and 12.11 +/- 2.08 pg/ml 5 min before the operation. We did not find statistically significant differences between the paired samples (t-value = -1.53, 2-tail probability p = 0.139). The increase in beta-endorphin could be the result of preoperative anxiety about the narcosis and surgery.
对23名因择期（非急诊）手术、腺样体切除术和/或扁桃体切除术入住儿童医院的儿童（16名男性，7名女性）的血浆中β-内啡肽和亮氨酸脑啡肽水平进行了测量。术前采集了两份血样。第一份样本在入院时采集；第二份在24小时后且在手术前5分钟采集，未进行术前用药或麻醉。样本经固相萃取后，采用放射免疫分析法测定β-内啡肽和亮氨酸脑啡肽水平。入院时β-内啡肽的平均浓度为11.77±9.36 pg/ml。24小时后，即在手术前5分钟，β-内啡肽的平均水平升至27.45±16.66 pg/ml。与24小时前相比，手术前5分钟β-内啡肽水平有统计学意义的升高（t值=-5.42）（双侧概率p<0.0001）。Wilcoxon配对符号秩检验也显示p<0.0001（Z=-4.197）。相比之下，亮氨酸脑啡肽水平保持不变。入院当天的平均值为11.44±1.49 pg/ml，手术前5分钟为12.11±2.08 pg/ml。我们未发现配对样本之间有统计学意义的差异（t值=-1.53，双侧概率p=0.139）。β-内啡肽的升高可能是术前对麻醉和手术焦虑的结果。
