Akoum A, Lemay A, Brunet C, Hébert J
Université Laval, Québec, Canada.
Fertil Steril. 1995 Feb;63(2):322-8. doi: 10.1016/s0015-0282(16)57363-4.
To evaluate in vitro the production of monocyte chemotactic protein-1 (MCP-1) by endometrial cells of patients with and without endometriosis.
Primary cultures of stromal and epithelial cells isolated from human endometrium were exposed during 24 hours to different cytokines. Monocyte chemotactic protein-1 secretion was analyzed in the culture medium.
Gynecology clinic and laboratories of endocrinology of reproduction and immunology.
Women presenting for infertility or pelvic pain in which endometriosis was diagnosed at laparoscopy (n = 6) and women presenting for tubal ligation without laparoscopic evidence of the disease (n = 6).
None.
De novo secretion of MCP-1 in the culture supernatant by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis after metabolic labeling with 35S-cysteine.
The incubation of endometrial epithelial cells of endometriosis women with either interleukin-1 beta or tumor necrosis factor-alpha resulted in the appearance of at least two and sometimes three bands having approximately 15, 13, and 9 kd molecular weights. These bands were identified as three distinct species of MCP-1 as their immunoprecipitation was prevented effectively in presence of an excess of cold MCP-1. In contrast, the endometrial epithelial cells of only one of six normal women produce significant levels of MCP-1 under the same stimulation conditions. The stromal cells of both groups of subjects do not secrete appreciable amounts of MCP-1 or only small quantities in two cases of endometriosis.
Monocyte chemotactic protein-1 secretion is upregulated in cytokine-stimulated endometrial epithelial cells of women having endometriosis as compared with normal women without evidence of the disease. Such a difference at the level of eutopic endometrial cell may have a significance in the physiopathology of endometriosis.
在体外评估患有和未患有子宫内膜异位症患者的子宫内膜细胞产生单核细胞趋化蛋白-1（MCP-1）的情况。
从人子宫内膜分离出的基质细胞和上皮细胞的原代培养物在24小时内暴露于不同的细胞因子。分析培养基中单核细胞趋化蛋白-1的分泌情况。
妇科诊所以及生殖内分泌与免疫学实验室。
因不孕或盆腔疼痛就诊且在腹腔镜检查中被诊断为子宫内膜异位症的女性（n = 6），以及因输卵管结扎就诊且腹腔镜检查未发现该疾病迹象的女性（n = 6）。
无。
在用35S-半胱氨酸进行代谢标记后，通过免疫沉淀和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳检测培养上清液中MCP-1的从头分泌情况。
用白细胞介素-1β或肿瘤坏死因子-α孵育子宫内膜异位症女性的子宫内膜上皮细胞，会出现至少两条且有时三条分子量约为15、13和9 kDa的条带。这些条带被鉴定为三种不同的MCP-1，因为在过量冷MCP-1存在时其免疫沉淀被有效阻止。相比之下，在相同刺激条件下，六名正常女性中只有一名的子宫内膜上皮细胞产生显著水平的MCP-1。两组受试者的基质细胞均不分泌可观量的MCP-1，或在两例子宫内膜异位症病例中仅分泌少量MCP-1。
与无该疾病证据的正常女性相比，细胞因子刺激的患有子宫内膜异位症女性的子宫内膜上皮细胞中单核细胞趋化蛋白-1的分泌上调。在位子宫内膜细胞水平的这种差异可能在子宫内膜异位症的病理生理学中具有重要意义。
