Zabel M, Seidel J, Kaczmarek A, Surdyk-Zasada J, Grzeszkowiak J, Górny A
Department of Histology and Embryology, Medical Academy, Poznań, Poland.
Histochemistry. 1994 Oct;102(4):323-7. doi: 10.1007/BF00269170.
The study was aimed at a morphological demonstration of calcitonin (CT) gene expression in cultured TT cells, or, more specifically, hybridocytochemical detection of CT mRNA and calcitonin gene-related peptide (CGRP) mRNA and ultrastructural localization of the two hormones. The TT cells originated from medullary carcinoma of human thyroid gland. Ultrastructural studies of TT cells demonstrated a well-developed rough endoplasmic reticulum, large Golgi apparatus and low number of secretory granules. Hybridocytochemical studies showed the presence of mRNAs for CT and CGRP in all TT cells. At the ultrastructural level, double immunolabelling demonstrated that the two hormones were always expressed together in the same secretory granules. Our results provide a significant addition to the biochemical studies performed up to now and indicate that all TT cells produce both mRNAs and both hormones in parallel.
本研究旨在对培养的TT细胞中降钙素（CT）基因表达进行形态学展示，或者更具体地说，对CT mRNA和降钙素基因相关肽（CGRP）mRNA进行杂交细胞化学检测，并对这两种激素进行超微结构定位。TT细胞源自人甲状腺髓样癌。对TT细胞的超微结构研究显示，其粗面内质网发达，高尔基体大，分泌颗粒数量少。杂交细胞化学研究表明，所有TT细胞中均存在CT和CGRP的mRNA。在超微结构水平上，双重免疫标记显示这两种激素总是在同一分泌颗粒中共同表达。我们的结果为迄今为止所进行的生化研究增添了重要内容，并表明所有TT细胞同时产生这两种mRNA和这两种激素。
