Bernan V S, Montenegro D A, Korshalla J D, Maiese W M, Steinberg D A, Greenstein M
Natural Products Research Section, Medical Research Division, American Cyanamid Company, Pearl River, NY.
J Antibiot (Tokyo). 1994 Dec;47(12):1417-24. doi: 10.7164/antibiotics.47.1417.
An actinomycete strain designated LL-31F508 was isolated from an intertidal sediment sample collected in Key West, Florida. Culture LL-31F508 was assigned to the Streptomyces genus based on the presence of LL-diaminopimelic acid (DAP) in the cell wall and observations of spiny spores using scanning electron microscopy (SEM). Excellent antimicrobial activity against Staphylococcus and Enterococcus spp. were detected in both the supernatant and cell extract samples from fermentations of culture LL-31F508. Production of antibiotic activity peaked at 48-50 hours and closely paralleled cell growth, during which time glucose was more rapidly assimilated than dextrin. A series of new antibiotics called the bioxalomycins was identified as the antibacterial products from fermentations of this culture. Fermentation conditions for production of bioxalomycin alpha differed substantially from those required for production of a related compound, naphthyridinomycin, by the reference culture Streptomyces lusitanus NRRL 8034.
从佛罗里达州基韦斯特采集的潮间带沉积物样本中分离出一株放线菌菌株，命名为LL - 31F508。基于细胞壁中存在LL - 二氨基庚二酸（DAP）以及使用扫描电子显微镜（SEM）观察到的带刺孢子，培养物LL - 31F508被归为链霉菌属。在培养物LL - 31F508发酵的上清液和细胞提取物样本中均检测到对葡萄球菌和肠球菌具有优异的抗菌活性。抗生素活性的产生在48 - 50小时达到峰值，且与细胞生长密切平行，在此期间葡萄糖比糊精更快速地被同化。一系列名为生物草霉素的新抗生素被鉴定为该培养物发酵产生的抗菌产物。生物草霉素α的发酵条件与参考培养物葡萄牙链霉菌NRRL 8034产生相关化合物萘啶霉素所需的条件有很大不同。
