Ma H W, Cheng J, Caddy B
Forensic Science Unit, University of Strathclyde, Glasgow, United Kingdom.
J Forensic Sci Soc. 1994 Oct-Dec;34(4):231-5. doi: 10.1016/s0015-7368(94)72925-x.
A simple and efficient method for extracting human genomic DNA from microsamples of blood has been developed. This method used sodium perchlorate, chloroform, polymerised silica gel and a dumbbell-shape tube, instead of proteinase K and phenol. The entire process took less than two hours, and high molecular weight DNA, in high yield and purity, was obtained from a few microlitres of human blood. DNA prepared in this way can be easily digested with restriction endonucleases and has been employed for DNA profiling and the polymerase chain reaction.
