Scherczinger C A, Bourke M T, Ladd C, Lee H C
Connecticut State Police Forensic Science Laboratory, Meriden, USA.
J Forensic Sci. 1997 Sep;42(5):893-6.
The implementation of convicted felon DNA databases by increasing numbers of forensic science laboratories has engendered the need for a quick, efficient, and cost-effective method for the isolation of DNA from liquid blood samples. Because of the large numbers of samples involved, the ideal method would combine high throughput capability with maximal yield, high quality, and minimal time. We have found that the QIAGEN QIAamp Blood Kit/Tissue Kit satisfy all of these requirements. This simple, low cost spin column procedure yields purified DNA of approximately 20-30 kb that can be used directly in PCR or other enzymatic reactions without further purification. We compared the QIAamp isolation procedure to the standard SDS-Proteinase K/organic extraction/microcon purification procedure currently used by many forensic laboratories. The QIAamp procedure consistently gave a two- to four-fold increased yield relative to the organic extraction procedure. The DNA obtained was of high molecular weight, exhibited little degradation, and was suitable for RFLP and PCR analyses. We have found QIAGEN's QIAamp DNA isolation procedure to be ideally suited for preparation of samples for DNA databasing.
越来越多的法医学实验室开始建立已定罪重罪犯的DNA数据库，这就需要一种快速、高效且经济高效的方法，用于从液态血样中分离DNA。由于涉及的样本数量众多，理想的方法应兼具高通量能力、最大产量、高质量以及最短时间。我们发现，QIAGEN公司的QIAamp血液/组织试剂盒满足所有这些要求。这种简单、低成本的离心柱方法可产生约20 - 30 kb的纯化DNA，无需进一步纯化即可直接用于PCR或其他酶促反应。我们将QIAamp分离方法与许多法医实验室目前使用的标准SDS - 蛋白酶K/有机提取/微浓缩纯化方法进行了比较。相对于有机提取方法，QIAamp方法的产量始终提高了两到四倍。获得的DNA分子量高，几乎没有降解，适用于RFLP和PCR分析。我们发现QIAGEN公司的QIAamp DNA分离方法非常适合为DNA数据库制备样本。
