Günther S, Herold J, Patzelt D
Institut für Rechtsmedizin, Julius-Maximilians-Universität, Würzburg, Germany.
Int J Legal Med. 1995;108(3):154-6. doi: 10.1007/BF01844828.
A simple method is described for the extraction of high quality DNA for PCR amplification. The DNA was extracted by using Chelex-100 ion exchange resin or a special cell lysis buffer containing proteinase K. For further purification the DNA was bound to silica in the presence of a chaotrophic agent. Hence it is possible to unlimitedly wash the bound DNA and inhibitory substances are removed. By using diatoms as a source of silicates, this method is very economical and can therefore be used as a routine method.
本文描述了一种用于提取高质量DNA以进行PCR扩增的简单方法。使用Chelex-100离子交换树脂或含有蛋白酶K的特殊细胞裂解缓冲液提取DNA。为进一步纯化，在离液剂存在下将DNA与二氧化硅结合。因此，可以无限次洗涤结合的DNA并去除抑制性物质。通过使用硅藻作为硅酸盐来源，该方法非常经济，因此可用作常规方法。
