Pernas M, Bartolomé J, Castillo I, Quiroga J A, Pardo M, Carreño V
Hepatology Unit, Fundación Jiménez Díaz, Madrid, Spain.
J Gen Virol. 1995 Feb;76 ( Pt 2):415-20. doi: 10.1099/0022-1317-76-2-415.
Several hepatitis C viruses (HCV) have been described. In this study, the prevalence of HCV subtypes 1a, 1b, 2a and 2b has been studied by means of specific PCR in 93 serum samples of Spanish patients. Among these, the HCV-1b subtype was the most frequently detected (62%). Complementary DNA fragments from non-structural region 3 (NS3) and 5 (NS5), obtained from serum samples of three Spanish patients, were amplified by PCR and the products were cloned and sequenced. Comparison of the sequence obtained with those previously published shows the highest homology (91.7% in NS3 and 91.8% in NS5) with the HCV-1b subtype. The incidence of the local variant was analysed among the HCV-1b-infected patients. In order to distinguish between the local and HCV-1b prototype subtype, a new specific PCR assay was designed using primers from NS5. In the majority of the 76 HCV-1b-infected patients, the local variant was the only subtype detected (53%). These findings support the existence of a local variant, belonging to the HCV-1b subtype.
已发现了几种丙型肝炎病毒（HCV）。在本研究中，通过特异性PCR对93份西班牙患者的血清样本进行了HCV 1a、1b、2a和2b亚型的流行率研究。其中，HCV-1b亚型的检测频率最高（62%）。从3名西班牙患者的血清样本中获得的非结构区3（NS3）和5（NS5）的互补DNA片段，通过PCR进行扩增，产物进行克隆和测序。将所得序列与先前发表的序列进行比较，结果显示与HCV-1b亚型的同源性最高（NS3中为91.7%，NS5中为91.8%）。分析了HCV-1b感染患者中本地变异株的发生率。为了区分本地变异株和HCV-1b原型亚型，使用来自NS5的引物设计了一种新的特异性PCR检测方法。在76例HCV-1b感染患者中，大多数患者检测到的唯一亚型是本地变异株（53%）。这些发现支持了属于HCV-1b亚型的本地变异株的存在。
