Navas S, Martín J, Quiroga J A, Castillo I, Carreño V
Department of Hepatology, Fundación Jiménez Díaz, Madrid, Spain.
J Virol. 1998 Feb;72(2):1640-6. doi: 10.1128/JVI.72.2.1640-1646.1998.
The degree of genetic variability in the hypervariable region 1 of hepatitis C virus (HCV) was analyzed by cloning and sequencing HCV genomes obtained in paired samples of serum, liver tissue, and peripheral blood mononuclear cells (PBMC) from four chronic hepatitis C patients. Genetic variability in serum was higher than in liver tissue or PBMC at the level of complexity (the number of different sequences obtained from each type of tissue) as well as at the level of genetic distance between all pairs of sequences within each tissue (compared by the Student t test; P < 0.001 for two patients and P < 0.01 for another). The spectrum of viral genomes differed among the three types of tissue, as shown by segregation of sequences according to their tissue of origin in phylogenetic analysis and by statistical analysis of mean genetic distances observed between sequences obtained from different tissues (P < 0.001), but sequences from liver tissue and PBMC were more closely related to each other than to those from serum.
通过对4例慢性丙型肝炎患者血清、肝组织和外周血单个核细胞（PBMC）配对样本中获得的丙型肝炎病毒（HCV）基因组进行克隆和测序，分析了HCV高变区1的基因变异程度。在复杂性水平（从每种组织类型获得的不同序列数量）以及每种组织内所有序列对之间的遗传距离水平上，血清中的基因变异高于肝组织或PBMC（通过Student t检验比较；2例患者P < 0.001，另2例患者P < 0.01）。如系统发育分析中根据病毒基因组的组织来源进行序列分离以及对从不同组织获得的序列之间观察到的平均遗传距离进行统计分析所示（P < 0.001），三种组织类型中的病毒基因组谱不同，但肝组织和PBMC中的序列彼此之间的关系比与血清中的序列更为密切。
