Someya K, Totsuka Y, Murakoshi M, Kitano H, Miyazawa T
Oleochemistry Research Center, Lion Corporation, Tokyo, Japan.
J Nutr Sci Vitaminol (Tokyo). 1994 Aug;40(4):315-24. doi: 10.3177/jnsv.40.315.
To study the antioxidant effect of palm fruit carotene on skin lipid peroxidation, the guinea pigs were orally fed ad libitum palm fruit carotene, beta-carotene, or vehicle emulsions, in which carotene (0.05%, w/w) was suspended in drinking water. After treatment of carotene for 12 weeks, animals were exposed to ultraviolet ray (UV), and squalene monohydroperoxide (SqOOH)/squalene (Sq) ratios in the skin lipid were analyzed using the chemiluminescence-HPLC method. Carotene accumulation was found in the skin of guinea pigs that were orally administered palm fruit carotene or beta-carotene. After UV irradiation, especially immediately after, the rise in the SqOOH/Sq ratio was effectively suppressed in both carotene-drinking groups in contrast with the control (carotene-untreated) group. An inverse correlation between the carotene content and the SqOOH/Sq ratio in the skin was also observed. The results suggested that palm fruit carotene intake prevents skin lipid peroxidation caused by UV irradiation.
为研究棕榈果胡萝卜素对皮肤脂质过氧化的抗氧化作用，给豚鼠随意口服棕榈果胡萝卜素、β-胡萝卜素或赋形剂乳剂，其中胡萝卜素（0.05%，w/w）悬浮于饮用水中。用胡萝卜素处理12周后，将动物暴露于紫外线（UV）下，采用化学发光-高效液相色谱法分析皮肤脂质中角鲨烯单氢过氧化物（SqOOH）/角鲨烯（Sq）的比值。在口服棕榈果胡萝卜素或β-胡萝卜素的豚鼠皮肤中发现有胡萝卜素蓄积。紫外线照射后，尤其是照射后即刻，与对照组（未用胡萝卜素处理）相比，两个胡萝卜素饮水组的SqOOH/Sq比值升高均得到有效抑制。还观察到皮肤中胡萝卜素含量与SqOOH/Sq比值呈负相关。结果表明，摄入棕榈果胡萝卜素可预防紫外线照射引起的皮肤脂质过氧化。
