Someya K, Totsuka Y, Murakoshi M, Kitano H, Miyazawa T
Oleochemistry Research Center, Lion Corporation, Tokyo, Japan.
J Nutr Sci Vitaminol (Tokyo). 1994 Aug;40(4):303-14. doi: 10.3177/jnsv.40.303.
To study the effect of palm fruit carotene intake on skin lipid peroxidation, hairless mice were given ad libitum palm fruit carotene, beta-carotene, or vehicle emulsions for 15 weeks in which the carotene (0.005%, w/w) was suspended in drinking water, and then their dorsal skin was exposed to ultraviolet ray (UV). The carotene content of the skin was increased by the oral intake of palm fruit carotene or beta-carotene. In carotene-drinking mice, before the UV irradiation, the amount of thiobarbituric acid-reacting substances (TBARS) in the skin was lower than that of control (carotene untreated) mice. The skin TBARS immediately after the UV irradiation was lower in carotene-treated mice than in control mice. At 24 h after irradiation, the skin TBARS of mice that orally received palm fruit carotene was lower than that of beta-carotene mice. Immediately after the UV irradiation, the skin carotene content transiently decreased but gradual recovery was observed at 48 h. In palm fruit carotene-treated mice, the rate of carotene recovery after UV irradiation was higher than in beta-carotene-treated mice. Retinol found in the skin had also decreased after UV irradiation, and recovered gradually in both carotene-drinking groups within 48 h. These results suggested that the carotene intake, especially palm fruit carotene, prevented skin lipid peroxidation in hairless mice.
为研究棕榈果胡萝卜素摄入对皮肤脂质过氧化的影响，将无毛小鼠随意给予棕榈果胡萝卜素、β-胡萝卜素或赋形剂乳剂，持续15周，其中胡萝卜素（0.005%，w/w）悬浮于饮用水中，然后将其背部皮肤暴露于紫外线（UV）下。通过口服棕榈果胡萝卜素或β-胡萝卜素可增加皮肤中的胡萝卜素含量。在饮用胡萝卜素的小鼠中，紫外线照射前，皮肤中硫代巴比妥酸反应物质（TBARS）的量低于对照组（未处理胡萝卜素）小鼠。紫外线照射后，胡萝卜素处理组小鼠皮肤中的TBARS立即低于对照组小鼠。照射后24小时，口服棕榈果胡萝卜素的小鼠皮肤TBARS低于β-胡萝卜素小鼠。紫外线照射后，皮肤中的胡萝卜素含量立即短暂下降，但在48小时时观察到逐渐恢复。在棕榈果胡萝卜素处理的小鼠中，紫外线照射后胡萝卜素的恢复率高于β-胡萝卜素处理的小鼠。紫外线照射后皮肤中的视黄醇也有所下降，且在两个饮用胡萝卜素组中均在48小时内逐渐恢复。这些结果表明，摄入胡萝卜素，尤其是棕榈果胡萝卜素，可预防无毛小鼠的皮肤脂质过氧化。
