Ishizuka S, Sonoyama K, Niki R
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
J Nutr Sci Vitaminol (Tokyo). 1994 Aug;40(4):363-9. doi: 10.3177/jnsv.40.363.
The frequency of apoptosis after treatment with 1,2-dimethylhydrazine (DMH) was counted in the descending colonic and rectal crypts of food-deprived and fed rats. Food-deprived or fed rats were subcutaneously injected with DMH (100 mg/kg body weight). Six hours after the injection, apoptotic cells were observed in crypt regions by light microscopy. The incidence of DMH-induced apoptosis in food-deprived rats was significantly higher than in fed rats. The incidence appeared to be higher in descending colon than in rectum. PAS staining revealed that DMH treatment lowered mucin secretion in crypts, which was substantially lowered by food deprivation. The effect of food deprivation on apoptosis induced by DMH may be due to the decrease in mucus barrier against DMH.
在饥饿和喂食的大鼠降结肠和直肠隐窝中，对用1,2 - 二甲基肼（DMH）处理后的细胞凋亡频率进行计数。将饥饿或喂食的大鼠皮下注射DMH（100mg/kg体重）。注射后6小时，通过光学显微镜在隐窝区域观察凋亡细胞。饥饿大鼠中DMH诱导的细胞凋亡发生率显著高于喂食大鼠。降结肠中的发生率似乎高于直肠。PAS染色显示，DMH处理降低了隐窝中的粘蛋白分泌，而饥饿则使其大幅降低。饥饿对DMH诱导的细胞凋亡的影响可能是由于对抗DMH的黏液屏障减少所致。
