Robins E W, Nelson R L
University of Illinois College of Medicine, Department of Surgery, Chicago 60612.
Anticancer Res. 1989 Jul-Aug;9(4):981-5.
Chlorophyllin (CHL) was tested for its effect on 1,2-dimethylhydrazine (DMH)-induced nuclear aberrations in rat colonic epithelium. Rats were given water containing CHL (1.5 mM) and food ad libitum. After 5 wks on this dietary regimen, the rats were given a single intraperitoneal injection of DMH (20 mg DMH base/kg body weight). The rats were sacrificed 18 hours later and their colons were removed. Ten consecutive crypts from the proximal and distal portion of each specimen were scored for nuclear aberrations and the karyorrhectic index (KI) was determined. Rats receiving CHL + DMH had significantly fewer nuclear aberrations (lower KI) in the colonic epithelium than rats given DMH alone. This implies that CHL, a known antimutagen, may have anticarcinogenic properties as well.
对叶绿酸（CHL）进行了测试，以研究其对1,2 - 二甲基肼（DMH）诱导的大鼠结肠上皮细胞核畸变的影响。给大鼠自由饮用含CHL（1.5 mM）的水并随意进食。在这种饮食方案下5周后，给大鼠单次腹腔注射DMH（20 mg DMH碱/ kg体重）。18小时后处死大鼠并取出其结肠。对每个标本近端和远端的十个连续隐窝进行核畸变评分，并测定核固缩指数（KI）。接受CHL + DMH的大鼠结肠上皮中的核畸变（KI较低）明显少于仅给予DMH的大鼠。这意味着已知的抗诱变剂CHL可能也具有抗癌特性。
